MONOCLONAL ANTI-BETA-GALACTOSIDASE ANTIB

Code: G8021-100UL D2-231

Application

Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used in:immunostaining and histochemistryimmunostaining and microscopyimmunohistochemistryimmu...


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Your Price
€563.00 EACH
€692.49 inc. VAT

Application

Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used in:immunostaining and histochemistryimmunostaining and microscopyimmunohistochemistryimmunofluorescence stainingenzyme linked immunosorbent assay(ELISA)immuno dot blot

Biochem/physiol Actions

β −galactosidase substrate is lactose. Synthetic substrates like o-nitrophenyl-β-d-galactopyranoside (oNPG) or p-nitrophenyl-β-d-galactopyranoside (pNPG) are used in enzymatic assays. It is also used as marker in studying gene expression. The use of monoclonal β -galactosidase anti- β -galactosidase (BGABG) soluble complex in the unlabeled antibody-enzyme method results in an intense signal with a very low background.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-β-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β −galactosidase is a E coli protein and is a tetramer with 1023 amino acids.

Immunogen

β-galactosidase from E. coli

Specificity

The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.

antibody formascites fluid
antibody product typeprimary antibodies
biological sourcemouse
cloneGAL-13, monoclonal
conjugateunconjugated
contains15 mM sodium azide
Gene InformationEscherichia coli ... lacZ(1036228), lacZ(1036228)Escherichia coli K12 ... Lacz(945006)
isotypeIgG1
Quality Level200
shipped indry ice
species reactivitybacteria
storage temp.−20°C
technique(s)immunocytochemistry: suitable, indirect ELISA: 1:2,000 using mouse primary antibody, bridging antibody and Escherichia coli β-galactosidase, dot blot: suitable using soluble β-galactosidase
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